Snowflake 3D Print Model

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3D Printing and Molecular Models

Department of Bioproducts & Biosystems Engineering

We have recently begun to utilize 3 dimensional printing technologies to produce different models of enzyme cofactors and protein structures. AMakerBot Replicator 2was recently purchased through the generous support of theBioTechnology Instituteat the University of Minnesota, and is housed in the Cargill Building on the St. Paul campus. This technology allows us to download pdb files from theProtein Data Bank, and using a range of software tools, we can finally import a completed file into theMakerWare Software, which then prepares a file that is used to print a scaled 3 dimensional model of the protein or cofactor

In support of this technology and to further promote what I feel is a potentially valuable educational tool, I have been building a number of these models and posting them toThingiverse. Anyone can go toThingiverseand download the files for printing on aMakerBot Replicator 2. I have already constructed a model of theFeMo-cofactor, which is the complex metallocenter found in the enzyme nitrogenase, an enzyme I studied for many years. Ive also printed two models of the simple proteinsCytochrome CandPlastocyanin. Ive also broken out the model paints and given them some color. I intend to make more models in the future. You can find my work underMoleculeMakeronThingiverse.

I hope to provide more details about how I constructed my models in the future. Initially, the files are downloaded from the Protein Data Bank as PDB files. I usually clean these up and remove any waters. These are then transferred to the program VMD, which is capable of producing an STL file, needed for import by the Makerware program. I usually select something specific from the protein to highlight. For example, I represent the heme molecule in cytochrome C as a CPK model, and then show the protein backbone. At this point, things get complicated, as VMD likes to layer the different representations on top of one another, which causes problems when trying to print. I am using the software Tinkercad to import the files, and then render a smaller file that removes all of the internal scaffolds, resulting in a much nicer print.Tinkercadrecently decided to close down their site, but was then purchased by Autodesk, and is now open again. I have also tried using SketchUp, but this software has a much steaper learning curve. I would like to print some more complicated models in the future, including a full model of nitrogenase and a full model of a photosynthetic reaction center. Stay tuned for more on this exciting new technology. I am also interested in hearing from others that are using this 3 dimensional printing technology in similar applications, as there may be additional software tools that I am missing.

Several of my protein models have been constructed as they have relevance to the projects we work on in the laboratory. This model of the wax ester synthase enzyme is based on a theoretical structure we utilize in the laboratory for this wax ester synthase enzyme. Since there is currently no published crystal structure for this enzyme, we have to use structures from related enzymes as a best guess for what the structure of our enzyme might look like. Using this model, we have predicted where specific residues might reside in our model, and have selected these for site specific mutagenesis studies. These experiments have produced several interesting results which we recently published in the journal Applied and Environmental Microbiology (2013). We also published a paper in 2012 that characterized the differences between five homologs of this enzyme in the same journal. This enzyme is a key focus of study in our laboratory, as it produces an ester product from a fatty acid and a fatty alcohol. If the alcohol is small enough, then the product would be similar to biodiesel. When the enzyme produces the product of the fatty acid and an alcohol such as ethanol, then the product has been termed microdiesel by another research group, as it is derived from a microorganism. This enzyme may someday be utilized to produce biodiesel from simple feedstocks such as sugars, or from complex biomass such as cellulose. Most ideally, we would like to produce it in a functioning phototroph, such as a cyanobacterium. The use of the models is thus another tool to be used in our research efforts.

One drawback to our previous 3D prints is the excessive effort that is required to remove the scaffolding to support the molecule during the printing process. This process can take several hours and results in very sore hands and potential damage to the model. Thus, I have been working recently to improve the models by incorporating our own structural supports. This results in less plastic utilized to print the model, and also drops down the time to remove the structure from several hours to a few minutes. It does require the use of a modeling knife to trim off the supports, but results in a much nicer looking final product.

To the left is the 3D model for theacyl carrier proteinas is looks following printing, while the image to the right shows the model 30 minutes later following removal of the supports and some cleanup with a modeling knife to soften the edges. This model is very strong and retains shape better than some of my previous models. I am currently constructing a model of the enzyme quercetinase as well as a model of the substrate. This is a nice enzyme to do modeling with, as the substrate is large and the structure was solved with a substrate bound. My hope is to build a model that students can use to insert the substrate component into the active site, which will assist in their understanding of how enzymes orient substrates to improve catalysis. We also utilize the quercetinase enzyme as part of a teach laboratory experiment in our BBE3013 course at UMN.

Department of Bioproducts and Biosystems Engineering

304 BioAgEng Bldg  1390 Eckles Avenue  St. Paul, MN 55108

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Check Out This Library of Over 200 3D Printed Dungeons Dragons Monsters

byScott J GrunewaldJan 15, 20163D Design3D Printed Art3D Printing

When Gary Gygax and Dave Arneson created the legendary Dungeons & Dragons (D&D) tabletop role playing game it is unlikely that they had any idea that it would become unquestionably the most popular RPG of all time. For over forty years the game has been a cultural touchstone for multiple generations of gamers and fans and has even endured and thrived in the age of the video game. That endurance has a lot to do with the themes of the game itself, thanks to its liberal use of modern fantasy mythology and iconography, but it is also due to the games ability to reinvent itself regularly. While not every new iteration of D&D or new game mechanic has been met well with fans, the evolution into a more visually-based game that allows users the option of using grid maps and mini figures has generally been a welcomed change.

Zavala carries all of his minis around in a giant tool box.

When avid D&D player and Shapeways userMiguel Zavalawas encouraged by his fiance to pick up 3D printing as a hobby, at first he wasnt sure it was for him. He had already worked with 3D printing while he was in college and never really pursued it after he graduated and got a job. But after finding that his local library offered 3D printing services he decided to go ahead and give it a try. As a fan of D&D, naturally, the first thing that he 3D sculpted was a Black Dragon miniature that could be used in his next game, and by the time it had finished printing he was hooked. In no time he had his ownPrintrbot Simpleat home, and decided to 3D sculpt and 3D print all of the hundreds of creatures from the gamesMonster Manual.

I actually started playing [D&D] when I was 17 and havent stopped since. While Ive grown up a plenty, D&D is the one thing that I will always feel like a kid around. Im just as excited now when I sit down and start rolling the dice as I was when I was a teen. I love this game, and it makes me feel absolutely fantastic knowing Im helping improve the experience for fellow gamers like myself, Zavala told me.

The 350 pageMonster Manualis one of the three core rule books that are required to run a D&D campaign, and it is packed full of just about every fantasy creature that you can think of, and quite a few that you probably couldnt (Im looking at you, Owlbear) so this was quite the challenging project that Zavala took on. So far hes 3D printed well over 200 of the monsters since he started back in the fall of 2014. The monsters range from role playing game mainstays like his incredibly detailed dragons to banshees, giants, demons, ghosts, specters and elementals. But hes also 3D printed plenty of the odd, weird and ridiculous creatures that the game has to offer, like the Flame Skull, which is exactly what it sounds like. Naturally hes printed what is probably one of the most iconic and weird D&D creature ever, the floating eyeball monster called a Beholder. And yes hardcore RPG fans, hes also 3D printed theDread Gazebo, because of course he would.

Zavala gets the reference materials for all of his designs directly from the source, the5th Edition Dungeons & Dragons Monster Manual. All of the 3D models are designed in Blender, which he then sends over to Cura to prepare for 3D printing. Aside from the first few dozen models, all of his miniatures were 3D printed on his Printrbot Simple in PLA. Zavala then hand paints all of the minis using standard acrylic model paints. Hes uploaded a huge gallery of all of his models ontoImgur, and all of the 3D models can bedownloaded from Shapeways. He originally had the models up on Thingiverse, but it turns out that Wizards of the Coast, the owners of Dungeons & Dragons, werent crazy about someone making their own D&D minis.

Wizards of the Coast requested my models taken down from Thingiverse. At first I was a bit surprised as I wasnt trying to make money off of this or anything. Thankfully when I reached out to them and explained my intentions, which were just to share my files so that fellow players could have just as much customization in their games as I have, they were pretty fair. They just preferred the models were on a site they had an agreement with. So thats why all my stuff is now on Shapeways. So long as I dont try to sell any of their IP I can post away here, Zavala explained.

Zavalas only goal with his project is is to give other D&D gamers the option to completely customize their own games and have as much fun playing as he has all these years. He plans to keep going and continue to design and 3D print new models regularly, until he runs out at least. But even with the two hundred plus figures that he has already made the sixteen year Army vet isnt even close to 3D printing all of the monsters, races and creatures found in the game, so I hope he has a lot of filament handy.  Let us know your thoughts on this story in the3D Printed Dungeons and Dragons forumon .

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Tagged with:3d printable miniaturesBeholderblenderCuraD&DDave ArnesondragonsDread Gazebodungeons & dragonsGary GygaximgurMiguel ZavalaMonster Manualrole playing gamesrole playing games. 3d printed miniaturesrpgshapewaystabletop miniaturesWizards of the Coast

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3D Printed Anatomy Models

At the forefront of both medicine and technology, these 3D Printed Anatomy Models are unlike anything on the market today. Created from a combination of MRI and CT scans of a real cadaver specimen, these models are printed to life using cutting-edge 3D printers and materials. Why use a 3D printed anatomy model? For one, the detail in each model is as close to life as anything that has ever been produced artificially. Not to mention, the ethical benefits of not working with actual human specimens or the mess and health risks associated with preserved specimen chemicals. Contact us today to order one of these incredible models!

3D printing is a revolutionary manufacturing process that works by building layers of material to create a 3-Dimensional object, and is virtually limitless in possibility. Using CT or MRI imaging data, we can model an actual human cadver and bring it to life in 3-Dimensions, or we can take your personal MRI and make a model of your exact anatomy, true to life and true to your unique anatomy. We are pleased to offer two lines of 3D printed anatomy models:

3DyourSCAN Models -Your Personal, Unique Anatomy Brought to Life

Have you ever wanted to see your own internal anatomy? Are you planning a complicated, high-risk surgery and need to know exactly what to expect when you enter the O.R.? Our 3DyourSCAN printed models are designed to give patients and physicians alike the power to hold patient-specific anatomical models in their hands, ensuring both medical accuracy and patient privacy. Take the mystery out of complex surgeries and instill confidence in your patients by showing them an exact replica of their anatomy before surgery. Before you go under the knife, insist your surgeon study your anatomy in a way like never before, by taking the MRI off the screen and putting it in his or her hands.

Human Cadaver Replicas -Uncompromised Anatomical Detail

These models are made from CT and MRI data of actual human specimens, and are 100% anatomically accurate to the specimen the model was made from. Every single detail, no matter how small, is brought to life in vivid detail and color. Gone are the days of ethically questionable human remains, as our human cadaver replicas leave no detail behind and, of course, do not require extensive use agreements or complicated preservation solutions. When you need the absolute best in anatomical accuracy, our Human Cadaver Replicas are second to none.

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RABBIT 3D Print Model

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Human skeleton 3D models

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case print 3d models

Taps and drills case print in place

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Jewelry Case 3D print modelThis is a foldable jewelry case with circular shape, originally designed for CNC production. It has been adjusted for 3D printing. The…

Pen case 3D print modelModel has three formatsi 1/4 ?MAX and OBJ file format are optimized filesi 1/4 ?Wireframes also optimizedi 1/4 ?STL file format is the source file, the source…

Pen case 3D print modelModel has three formatsi 1/4 ?MAX and OBJ file format are optimized filesi 1/4 ?Wireframes also optimizedi 1/4 ?STL file format is the source file, the source…

Pen case 3D print modelModel has three formatsi 1/4 ?MAX and OBJ file format are optimized filesi 1/4 ?Wireframes also optimizedi 1/4 ?STL file format is the source file, the source…

Pen case 3D print modelModel has three formatsi 1/4 ?MAX and OBJ file format are optimized filesi 1/4 ?Wireframes also optimizedi 1/4 ?STL file format is the source file, the source…

Pen case 3D print modelModel has three formatsi 1/4 ?MAX and OBJ file format are optimized filesi 1/4 ?Wireframes also optimizedi 1/4 ?STL file format is the source file, the source…

Pen case 3D print modelModel has three formatsi 1/4 ?MAX and OBJ file format are optimized filesi 1/4 ?Wireframes also optimizedi 1/4 ?STL file format is the source file, the source…

Pen case 3D print modelModel has three formatsi 1/4 ?MAX and OBJ file format are optimized filesi 1/4 ?Wireframes also optimizedi 1/4 ?STL file format is the source file, the source…

Pen case 3D print modelModel has three formatsi 1/4 ?MAX and OBJ file format are optimized filesi 1/4 ?Wireframes also optimizedi 1/4 ?STL file format is the source file, the source…

Pen case 3D print modelModel has three formatsi 1/4 ?MAX and OBJ file format are optimized filesi 1/4 ?Wireframes also optimizedi 1/4 ?STL file format is the source file, the source…

Pen case 3D print modelModel has three formatsi 1/4 ?MAX and OBJ file format are optimized filesi 1/4 ?Wireframes also optimizedi 1/4 ?STL file format is the source file, the source…

Pen case 3D print modelModel has three formatsi 1/4 ?MAX and OBJ file format are optimized filesi 1/4 ?Wireframes also optimizedi 1/4 ?STL file format is the source file, the source…

Pen case 3D print modelModel has three formatsi 1/4 ?MAX and OBJ file format are optimized filesi 1/4 ?Wireframes also optimizedi 1/4 ?STL file format is the source file, the source…

Pen case 3D print modelModel has three formatsi 1/4 ?MAX and OBJ file format are optimized filesi 1/4 ?Wireframes also optimizedi 1/4 ?STL file format is the source file, the source…

Pen case 3D print modelModel has three formatsi 1/4 ?MAX and OBJ file format are optimized filesi 1/4 ?Wireframes also optimizedi 1/4 ?STL file format is the source file, the source…

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